Heterologous microsatellite-based genetic diversity in blue mussel (Mytilus chilensis) and differentiation among localities in southern Chile

摘要: Mussels (Mytilus spp.) are one of the most cultivated and commercialized bivalves in southern Chile; culture is currently supplied almost entirely from wild-caught seed obtained relatively few collection centers. The genetic diversity differentiation blue mussel Chile was investigated by sampling six locations: natural bank five Nine polymorphic microsatellite (SSR) loci were genotyped (Mgu1, Mgu3, MT203, MT282, Mg15, Mg56, Med737, MIT02 MGE005). We found 75 different alleles, which private alleles. Of analyzed loci, 45 54 tests performed deviated Hardy-Weinberg equilibrium after sequential Bonferroni correction (P < 0.05), revealing significant heterozygote deficiencies. information content (PIC) ranged 0.322 (MGE005) to 0.893 (Mgu1). Despite long distance between some sites (up 1360 km), among low (FST = 0.043, P 0.0001). Bayesian cluster analysis (STRUCTURE) indicated two probable clusters, while non-parametric (AWclust) identified four clusters. Both analyses showed a high level admixture within Our results indicate that mussels show lower than other countries, inbreeding levels, limited locations.