A strong intronic enhancer element of the EGFR gene is preferentially active in high EGFR expressing breast cancer cells.
作者： Jane M. McInerney , Melissa A. Wilson , Kate J. Strand , Susan A. Chrysogelos
DOI: 10.1002/1097-4644(20010315)80:4<538::AID-JCB1008>3.0.CO;2-2
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摘要: Hormone-independent human breast cancer is characterized by estrogen receptor (ER) loss and the acquisition of high epidermal growth factor (EGFR) levels. Despite tendency for an inverse correlation between EGFR ER, a strong prognostic indicator poor survival rate independent ER status suggesting that overexpression important step in progression to independence. We have previously shown several DNase I hypersensitive sites which correspond potential regulatory regions reside within gene first intron exclusively hormone-independent cells. CAT assays investigating transcriptional activity indicate 140 bp region has enhancer ability specifically these The DNA–protein interaction occurs this was localized 35 bp displayed enhancer-like same Furthermore, protein binds seems be ubiquitous cell lines tested but higher abundance expressing Identifying specific elements involved up–regulation could lead development therapies preventing treating estrogen-independent cancer. J. Cell. Biochem. 80:538–549, 2001. © 2001 Wiley-Liss, Inc.
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