Gene dosage PCR and fluorescence in situ hybridization reveal low frequency of egfr amplifications despite protein overexpression in invasive breast carcinoma

摘要: The aim of this study was to assess the frequency egfr whole gene and CA intron repeat amplification in invasive breast cancer as a mechanism for epidermal growth factor receptor (EGFR) protein overexpression. By means tissue microarrays, overexpression were assessed 222 cases by immunohistochemistry FISH, respectively. First Taqman RT-PCR. With FISH RT-PCR, 4.7 6.3% showed first amplification, Amplification dosage varied between two- four-fold immunohistochemistry, 17.3% EGFR There low correlation different methods. In all, 2.9% both 90.3% negative both. Nearly 20% with immunohistochemical only 2.2% that on such amplification. 13% 1.6% Of overexpression, 4 (25%) either or conclusion, amplifications are rare explain about 12.5% cases. is another important explaining 18.7% However, since 75% lack these amplifications, other expression regulating mechanisms must be considered.