MiR-183 overexpression inhibits tumorigenesis and enhances DDP-induced cytotoxicity by targeting MTA1 in nasopharyngeal carcinoma.

作者: Guanghui Wang , Shujing Wang , Congying Li

DOI: 10.1177/1010428317703825

关键词:

摘要: MicroRNA 183 (miR-183) was identified to be downregulated in nasopharyngeal carcinoma spheroids and served as a tumor suppressor carcinoma. However, the regulatory mechanism of miR-183 its role cisplatin (DDP) resistance cells are still unclear. The expression metastasis-associated protein 1 at messenger RNA levels tissues evaluated using quantitative reverse transcription real-time polymerase chain reaction western blotting, respectively. CNE1 CNE2 were transfected with mimic, inhibitor, pcDNA-metastasis-associated 1, or respective controls. effects overexpression on cell proliferation, invasion, DDP-induced apoptosis detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell invasion flow cytometry analysis, Luciferase reporter assay performed explore whether directly targeted 1. Xenograft experiment applied confirm biological function vivo. MiR-183 negatively correlated expression. Ectopic markedly suppressed proliferation strikingly enhanced cells, whereas partially reversed these effects. demonstrated that direct target miR-183. regulated both levels. indicated repressed growth improved cytotoxicity inhibited tumorigenesis targeting carcinoma, contributing development novel therapeutic approaches for treatment clinical patients.

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