Human chorionic gonadotropin regulates endothelial cell responsiveness to interleukin 1 and amplifies the cytokine-mediated effect on cell proliferation, migration and the release of angiogenic factors.

摘要: Problem Successful embryonic implantation requires an appropriate communication network between the embryo and its near environment within site. Herein, we examined whether human chorionic gonadotropin (hCG), major signal, targets endothelial cells regulate their responsiveness to interleukin 1 (IL1), one of earliest signals released by cells. Method study Human microvascular cell proliferation migration following exposure various concentrations hCG and/or IL1B for different time periods were analyzed BrdU incorporation wound healing assays. The expression soluble (s) membrane-bound (mb) IL1 receptors (IL1Rs), IL1R antagonist (IL1RN), luteinizing hormone/choriogonadotropin receptor (LHCGR), IL8 was determined real-time PCR, Western blot, ELISA. Results Cell increased in response further presence hCG. up-regulated both signaling IL1R1 inhibitory IL1R2, while adding significantly downregulated IL1R2. This translated into secretion IL8, which inhibited where IL1R2 overexpressed. Conclusions These findings reveal a new mechanism may target directly stimulate angiogenesis favor growth.