Inhibition by zinc of rhinovirus protein cleavage: interaction of zinc with capsid polypeptides.

摘要: Zinic ions rapidly inhibit virus production in HeLa cells infected with human rhinovirus type 1A and lead to the accumulation of precursor polypeptides. The degree which cleavage these precursors is inhibited directly dependent on quantity cell-associated zinc. Proteolysis resumes after removal zinc-containing medium, accumulated viral are cleaved predominantly stable stabilized at lowest zinc levels those that contain capsid protein sequences. Furthermore, added bound capsids prevents them from forming crystals. Zinc-resistant mutants display antigenic alterations coat proteins. These results suggest complexes proteins alters so they cannot function as substrates for proteases or reactants assembly particles.