Rapid protein kinase D translocation in response to G protein-coupled receptor activation. Dependence on protein kinase C.

摘要: Abstract Protein kinase D (PKD)/protein C (PKC) μ is a serine/threonine protein that can be activated by physiological stimuli like growth factors, antigen-receptor engagement and G protein-coupled receptor (GPCR) agonists via phosphorylation-dependent mechanism requires PKC activity. In order to investigate the dynamic mechanisms associated with GPCR signaling, intracellular translocation of green fluorescent protein-tagged PKD was analyzed real-time visualization in fibroblasts epithelial cells stimulated bombesin, agonist. We found bombesin induced rapidly reversible plasma membrane PKD, an event divided into two distinct mechanistic steps. The first step, which exclusively mediated cysteine-rich domain N terminus involved its from cytosol membrane. second i.e. rapid reverse cytosol, required catalytic surprisingly These findings provide evidence for novel coordinates activation response bombesin-induced activation.