RNA-peptide fusions for the in vitro selection of peptides and proteins
作者： R. W. Roberts , J. W. Szostak
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摘要: Covalent fusions between an mRNA and the peptide or protein that it encodes can be generated by in vitro translation of synthetic mRNAs carry puromycin, a peptidyl acceptor antibiotic, at their 3′ end. The stable linkage informational (nucleic acid) functional (peptide) domains resulting joint molecules allows specific to enriched from complex mixture based on properties its encoded peptide. Fusions myc epitope have been pool random sequence mRNA-peptide immunoprecipitation. RNA-peptide should provide additional route selection directed evolution proteins.
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