Overexpression of adenylate cyclase-associated protein 1 is associated with metastasis of lung cancer.

摘要: Lung cancer ranks first in both prevalence and mortality rates among all types of cancer. Metastasis is the main cause treatment failure. Biomarkers are critical to early diagnosis prediction monitoring progressive lesions. Several biomarkers have been identified for lung but none routinely used clinically. The present study assessed diagnostic prognostic value cyclase-associated protein 1 (CAP1) CAP1 mRNA abundance content were determined by real-time PCR western blot analysis and/or immunostaining biopsy specimens (24 neoplastic 6 non-neoplastic) freshly collected at surgical resection, 82 pathologically banked cultured non-invasive (95-C) invasive (95-D) cells. Multivariate regression was performed correlate immunoreactive signal with type stage. In vitro cell migration determine effect RNA interference-mediated gene silencing on invasiveness 95-D These analyses collectively demonstrated that: i) significantly higher compared non-neoplastic metastatic non-metastatic not different between adenocarcinoma squamous carcinoma; ii) stronger cells 95-C cells; iii) adequately attenuated capacity vitro. findings suggest that overexpression cells, particularly stage, may significant clinical implications as a diagnostic/prognostic factor