Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer

摘要: CpG island hypermethylation and global genomic hypomethylation are common epigenetic features of cancer cells. Less attention has been focused on histone modifications in We characterized post-translational to H4 a comprehensive panel normal tissues, cell lines primary tumors. Using immunodetection, high-performance capillary electrophoresis mass spectrometry, we found that cells had loss monoacetylated trimethylated forms H4. These changes appeared early accumulated during the tumorigenic process, as showed mouse model multistage skin carcinogenesis. The losses occurred predominantly at acetylated Lys16 Lys20 residues were associated with DNA repetitive sequences, well-known characteristic Our data suggest monoacetylation trimethylation is hallmark human tumor