Peripheral but not central leptin treatment increases numbers of circulating NK cells, granulocytes and specific monocyte subpopulations in non-endotoxaemic lean and obese LEW-rats.
作者： Philipp Haas , Rainer H. Straub , Sammy Bedoui , Heike Nave
DOI: 10.1016/J.REGPEP.2008.05.004
关键词:
摘要: Leptin, a hormone mainly generated by adipocytes, acts centrally in the hypothalamus to regulate body weight and energy expenditure. However, there is strong evidence that leptin also involved cell-mediated immunity cytokine crosstalk. In present study effects of diet-induced obesity central peripheral treatment on leukocyte subsets production was investigated. Leptin injected either intravenously (i.v.) or intracerebroventricularly (i.c.v.) male endotoxaemic vehicle-treated healthy LEW-rats. Numbers blood were analysed FACS cytokines (TNF-alpha IL-6) ELISA. Results showed rather than able significantly increase numbers granulocytes, NK cells monocytes. Three-colour staining revealed ED9(+) monocytes most likely due mobilization two distinct monocyte subsets, predominantly ED9(+)CD4(-)NKR-P1A(+) ED9(+)CD4(+)NKR-P1A(+). ELISA analysis elevated TNF-alpha levels obese animals compared their lean littermates, while IL-6 failed show notable changes. conclusion, data application induces nutrition- application-site dependent circulating cells, granulocytes specific subsets.
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