Alterations of the glucose metabolism in a triose phosphate isomerase-negative Saccharomyces cerevisiae mutant.

摘要: The absence of triose phosphate isomerase activity causes an accumulation only one the two trioses, dihydroxyacetone phosphate, and this produces a shift in final product glucose catabolism from ethanol to glycerol (Compagno et al., 1996). Alterations metabolism imposed by deletion TPI1 gene Saccharomyces cerevisiae were studied batch continuous cultures. Δtpi1 null mutant was unable grow on as sole carbon source. addition or acetate media containing glucose, but also raffinose galactose, relieved effect cultivation, suggesting that Crabtree is not primary cause for mutant's impaired growth glucose. energy source like formic acid restored utilization, NADH/energy shortage could be amount production represent good indicator fraction channelled through glycolysis. Data obtained cultures mixed substrates indicated different contributions glycolysis gluconeogenesis, well HMP pathway, utilization may occur relation present media. Copyright © 2001 John Wiley & Sons, Ltd.