The effect of p21 antisense oligodeoxynucleotides on the radiosensitivity of nasopharyngeal carcinoma cells with normal p53 function.

摘要: Abstract Objective p21 WAF1/CIP1 is transcriptionally activated by p53 and required for G 1 to S phase progression. plays a critical role in DNA repair after damage. Thus, cells with defective may result an enhancement of radiation induced apoptosis improved radiosensitivity. We tested the hypothesis that antisense oligodeoxynucleotides (p21 AS ODNs) can be used reduce expression level increase radiosensitivity CNE-1-wtp53 nasopharyngeal carcinoma cell line normal function. Methods materials The random control RD were synthesized. ODNs sequence: 5′-TGTCATGCTGGTCTGCCGCC-3′; 5′-CCGGTGAACGAGCGAGCACA-3′. transfected into line. protein levels P21 evaluated using Western blotting analysis. Cell cycle progression apoptotic assessed flow cytometric clonogenic survival assay was performed determine fraction. parameters D 0 , Dq, N single-hit multitarget model α β / SF 2 linear-quadratic calculated. BALB/c nude mice investigate effect on xenografts vivo. Results detected mainly plasma fluorescence microscopy investigation. dramatically decreased amount increased than irradiation. percentage arrest 24 h radiation, then 48 h radiation. values value cells. inhibition rate tumor exposed X ray 10 Gy alone 39.1%, while it 51.4% injected before exposure Unfortunately, there no significant difference between these two groups ( P Conclusion Antisense led expression, loss arrest, vitro inhibited growth become promising strategy enhance