Simultaneous quantitative analysis of FcγRI (CD64) expression on neutrophils and monocytes: A new, improved way to detect infections
作者： Jari Nuutila , Ulla Hohenthal , Iina Laitinen , Pirkko Kotilainen , Allan Rajamäki
DOI: 10.1016/J.JIM.2007.09.002
关键词:
摘要: We performed simultaneous quantitative flow cytometric analysis of neutrophil and monocyte FcgammaRI (CD64) in 289 hospitalized febrile patients. Microbiological evaluation or clinical diagnosis confirmed bacterial (n=89) viral (n=46) infection 135 Patient data were compared with from 60 healthy controls. The average number on the surfaces both neutrophils monocytes was significantly increased patients infections, to Furthermore, we describe a novel marker infection, designated 'CD64 score point', which incorporates expressed monocytes, 94% sensitivity 98% specificity distinguishing between infections By contrast, expression displayed poor (73% 52%) (65% levels did not differ systemic (sepsis), local, clinically diagnosed infections. In summary, our results clearly show that is useful but cannot be applied for differential local
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