Expression of the hematopoietic growth factor receptor FLT3 (STK-1/Flk2) in human leukemias.
作者： CE Carow , M Levenstein , SH Kaufmann , J Chen , S Amin
DOI: 10.1182/BLOOD.V87.3.1089.BLOODJOURNAL8731089
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摘要: Normal expression of the hematopoietic growth factor receptor FLT3 (STK-1@Flk2) is limited to CD34+ stem/progenitor cells. We have evaluated by RNase protection assay and Western blotting in 161 primary bone marrow (BM) samples from patients with leukemia. RNA was found be expressed at a higher level than normal BM controls 33 B-lineage acute leukemias, 11 12 myeloid leukemias (AMLs), 3 T-cell (T-ALLs). Expression also observed some cases blast crisis CML. The signal resulted on leukemic blasts, not caused increased cells samples. To determine if protein overexpressed, proteins were extracted screened anti-FLT3 antisera. detected controls, but 14 ALLs, 36 41 AMLs, 1 4 T-ALLs. Stimulation patient ligand autophosphorylation receptor, suggesting functional these These data show that are aberrantly AML ALL CD34 no longer synchronous, suggest possibility overexpression could play role survival and/or proliferation malignant clones lymphoid leukemias.
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