Synaptic transmission in the hippocampus: Critical role for glial cells

摘要: The importance of glial cells in controlling the neuronal microenvironment has been increasingly recognized. We now demonstrate that play an integral role hippocampal synaptic transmission by using glial-specific metabolic blocker fluoroacetate (FAC) to selectively inhibit cell function. FAC inhibits evoked intracellular postsynaptic potentials (PSPs; IC50 = 39 μM) as well population PSPs (IC50 65 field CA1 guinea pig slice. Spontaneous is concurrently decreased. These effects are time and dose dependent. ATP concentrations but not elements also significantly reduced with treatment. Simultaneous application substrate isocitrate prevents both reduction decrease PSPs. Given taken up glia, these data further support a specific action FAC. Additionally, no peak responses iontophoretically applied glutamate unchanged. However, decay iontophoretic prolonged following treatment suggesting inhibition uptake may contribute FAC-induced depression potentials. results show, for first time, critical maintenance suggest modulation efficacy. © 1994 Wiley-Liss, Inc.