Controlled intramyocardial release of engineered chemokines by biodegradable hydrogels as a treatment approach of myocardial infarction.

摘要: Myocardial infarction (MI) induces a complex inflammatory immune response, followed by the remodelling of heart muscle and scar formation. The rapid regeneration blood vessel network system attraction hematopoietic stem cells is beneficial for function. Despite important role chemokines in these processes, their use clinical practice has so far been limited availability over long time-span vivo. Here, method presented to increase physiological at site injury defined simultaneously control release using biodegradable hydrogels. Two different hydrogels were implemented, fast degradable hydrogel (FDH) delivering Met-CCL5 24 hrs slow (SDH) gradual protease-resistant CXCL12 (S4V) 4 weeks. We demonstrate that time-controlled Met-CCL5-FDH (S4V)-SDH suppressed initial neutrophil infiltration, promoted neovascularization reduced apoptosis infarcted myocardium. Thus, we able significantly preserve cardiac function after MI. This study demonstrates time-controlled, biopolymer-mediated delivery represents novel feasible strategy support endogenous reparatory mechanisms MI may compliment cell-based therapies.