The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
作者： J Wade Harper , Guy R Adami , Nan Wei , Khandan Keyomarsi , Stephen J Elledge
DOI: 10.1016/0092-8674(93)90499-G
关键词:
摘要: The cyclin-dependent kinase Cdk2 associates with cyclins A, D, and E and has been implicated in the control of the G1 to S phase transition in mammals. To identify potential Cdk2 regulators, we have employed an improved two-hybrid system to isolate human genes encoding Cdk-interacting proteins (Cips). CIP1 encodes a novel 21 kd protein that is found in cyclin A, cyclin D1, cyclin E, and Cdk2 immunoprecipitates. p21 CIP1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk2, cyclin E …
ceek.jp
elsevier.com参考文章(46)
Matthew Meyerson, Greg H Enders, Chin-Lee Wu, LK Su, C Gorka, C Nelson, Ed Harlow, LH Tsai, A family of human cdc2-related protein kinases. The EMBO Journal. ,vol. 11, pp. 2909- 2917 ,(1992) , 10.1002/J.1460-2075.1992.TB05360.X
J-Y Kato, H Matsushime, SW Hiebert, ME Ewen, CJ Sherr, Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent kinase CDK4. Genes & Development. ,vol. 7, pp. 331- 342 ,(1993) , 10.1101/GAD.7.3.331
L. L. Parker, S. Atherton-Fessler, M. S. Lee, S. Ogg, J. L. Falk, K. I. Swenson, H. Piwnica-Worms, Cyclin promotes the tyrosine phosphorylation of p34cdc2 in a wee1+ dependent manner. The EMBO Journal. ,vol. 10, pp. 1255- 1263 ,(1991) , 10.1002/J.1460-2075.1991.TB08067.X
Robert S. Sikorski, Jef D. Boeke, In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast. Methods in Enzymology. ,vol. 194, pp. 302- 318 ,(1991) , 10.1016/0076-6879(91)94023-6
S.J. Elledge, M.R. Spottswood, A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1. The EMBO Journal. ,vol. 10, pp. 2653- 2659 ,(1991) , 10.1002/J.1460-2075.1991.TB07808.X
M.J. Solomon, J.W. Harper, J. Shuttleworth, CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15. The EMBO Journal. ,vol. 12, pp. 3133- 3142 ,(1993) , 10.1002/J.1460-2075.1993.TB05982.X
Arthur B. Pardee, Shanchuan Zhao, Andrew H. Levin, Qing Ping Dou, Cyclin E and cyclin A as candidates for the restriction point protein. Cancer Research. ,vol. 53, pp. 1493- 1497 ,(1993)
C Chen, H Okayama, High-efficiency transformation of mammalian cells by plasmid DNA. Molecular and Cellular Biology. ,vol. 7, pp. 2745- 2752 ,(1987) , 10.1128/MCB.7.8.2745
J. Rosenblatt, Y. Gu, D. O. Morgan, Human cyclin-dependent kinase 2 is activated during the S and G2 phases of the cell cycle and associates with cyclin A Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 2824- 2828 ,(1992) , 10.1073/PNAS.89.7.2824
M. Mendenhall, An inhibitor of p34CDC28 protein kinase activity from Saccharomyces cerevisiae Science. ,vol. 259, pp. 216- 219 ,(1993) , 10.1126/SCIENCE.8421781