Brain proteomics of Anopheles gambiae.
作者： Sutopa B Dwivedi , Babylakshmi Muthusamy , Praveen Kumar , Min-Sik Kim , Raja Sekhar Nirujogi
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摘要: Anopheles gambiae has a well-adapted system for host localization, feeding, and mating behavior, which are all governed by neuronal processes in the brain. However, there no published reports characterizing brain proteome to elucidate signaling mechanisms vector. To this end, large-scale mapping of An. was carried out using high resolution tandem mass spectrometry, revealing repertoire >1800 proteins, 15% could not be assigned any function. A large proportion identified proteins were predicted involved diverse biological including metabolism, transport, protein synthesis, olfaction. This study also led identification 10 GPCR classes govern sensory pathways mosquitoes. Proteins metabolic neural processes, chromatin modeling, synaptic vesicle transport associated with transmission predominantly expressed Proteogenomic analysis expanded our findings 15 novel genes 71 cases gene refinements, subset validated RT-PCR sequencing. Overall, offers valuable insights into physiology vector that possibly open avenues intervention strategies malaria future.
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