Co-expression of Erns and E2 genes of classical swine fever virus by replication-defective recombinant adenovirus completely protects pigs against virulent challenge with classical swine fever virus.
作者： Yongke Sun , Yuai Yang , Huanli Zheng , Dongmei Xi , Mingxing Lin
DOI: 10.1016/J.RVSC.2012.09.012
关键词:
摘要: The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry reduction losses involved CSF outbreaks. Erns and E2 genes classical swine fever virus (CSFV), which encode two main protective glycoproteins from "Shimen" strain CSFV, were combined inserted into replication-defective human type-5 named rAd-Erns-E2. Nine pigs randomly assigned three treatment groups (three each group) including rAd-Erns-E2, hAd-CMV control DMEM control. Intramuscular vaccination with 2×10(6) TCID(50) rAd-Erns-E2 administered times an interval 21 days. At 42 days post inoculation, all challenged lethal dose 1×10(3) strain. Observation clinical signs made existence RNA detected. Animals showed severe symptoms euthanized 7 10 after challenge. However, no adverse observed vaccinated administration even Neither nor pathological changes detected tissues interest above pigs. These results implied that recombination carrying Erns-E2 could be prevent fever.
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