Intrathecal treatment of C6 glioma leptomeningeal metastasis in Wistar rats with interlenkin-2

摘要: The efficacy of intrathecal treatment leptomeningeal metastasis (LM) with interleukin-2 (IL-2) was evaluated in an animal model using Wistar rats inoculated intracisternally 107 C6 glioma cells. Prior to the vivo experiments antiproliferative effects human IL-2, and murine IFN-γ TNF-α which are cytokines induced by IL-2 were tested a colony forming assay. Only caused dose-dependent inhibition formation. Twelve animals treated either 105 IU or control medium on day 0, 2, 5 after tumor cell inoculation. Both sham-treated developed LM symptom-free survival 7 9 days. There no significant difference between untreated regarding time onset symptoms pattern growth. Infiltration tissue ED-1+ monocytes macrophages, CD8+ lymphocytes, however, slightly increased animals. In second experiment 4 non tumor-bearing injected single dose IL-2. These also showed enhanced infiltration lymphocytes compared controls. We conclude that application high-dose although eliciting slight immune reaction within leptomeninges does not inhibit growth prolong our LM. results raise doubt about clinical patients