Comet assay as a novel approach for studying DNA damage in focal cerebral ischemia: differential effects of NMDA receptor antagonists and poly(ADP-ribose) polymerase inhibitors.
作者： Lisa Giovannelli , Andrea Cozzi , Ilaria Guarnieri , Piero Dolara , Flavio Moroni
DOI: 10.1097/00004647-200206000-00008
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摘要: The single-cell gel electrophoresis (comet assay) was used to evaluate the possibility of detecting single-strand breaks brain DNA in early phase ischemia. Four hours after occlusion middle cerebral artery (MCAO) rats, percentage migrating into comet tail (indicating presence breaks) increased from 11.4 ± 4.70 34.7 9.2 (means SD) caudate and 9.9 4.3 42.8 14.1 cortex. Interestingly, a subpopulation cells exhibiting higher resistance ischemic insult present putamen, but not Administration MK801, an N-methyl-d-aspartate (NMDA) glutamate receptor antagonist, (1 mg/kg subcutaneously, 10 minutes before MCAO), reduced ischemia-induced infarct volume, suggesting that excessive stimulation NMDA receptors contributes formation both damage volume. In contrast, DPQ, inhibitor poly(ADP-ribose) polymerase (PARP) (10 intraperitoneally, 2...
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