Association between 8-hydroxy-2′-deoxyguanosine formation and DNA strand breaks mediated by copper and iron

摘要: Abstract Oxidative DNA damage is involved in diverse biological phenomena and consists of several kinds lesions, mainly, strand breaks, base modifications, DNA-protein crosslinking. However, little known about the existence a chemical relationship among them or ratio by which these different types lesions are produced. In present study we investigated whether exists between breakage modification. We selected cupric [Cu(II)] ferric [Fe(III)] ions for this because transition metals active catalysts vivo. Supercoiled plasmid pZ189 was treated with Cu(II) Fe(III) presence reducing agents. measured each sample both number single-strand breaks (SSB) quantitative electrophoresis amount modified base, 8-hydroxy-2′-deoxyguanosine (8-OHdG) HPLC simultaneous electrochemical (EC) spectrophotometric detection. The SSBs produced linearly related to 8-OHdG present. regression as function expressed equation [SSBs] = b × [8-OHdG], where 1.7, 2.0, 2,7, 9.4, H 2 O , l -cysteine -ascorbate, respectively. linear correlation observed production SSB mediated suggests that products may arise via common mechanism could allow an easier more precise estimation breakage.