Glomerular expression of the ATP-sensitive P2X7 receptor in diabetic and hypertensive rat models

摘要: Glomerular expression of the ATP-sensitive P2X7 receptor in diabetic and hypertensive rat models. Background. The molecular identification characterization adenosine triphosphate (ATP)-sensitive family P2 receptors is comparatively new. There are two main subgroups, each with several subtypes widespread tissue distribution, including kidney. A unique member P2X subgroup ATP-gated ion channel P2X7, which on activation can cause cell blebbing, cytokine release, death by necrosis or apoptosis. We report this normal kidney chronic models glomerular injury: streptozotocin-induced (STZ) diabetes ren-2 transgenic (TGR) hypertension. Methods. At different time points these models, we used a polyclonal antibody to immunohistochemistry determine its distribution. also Western blotting real-time polymerase chain reaction (PCR) detect changes protein mRNA expression, respectively. Results. found only low-level immunostaining for kidney, but intense glomeruli kidneys from animals at 6 9 weeks, 12 weeks. In animals, PCR demonstrated ∼tenfold increase relative control, confirmed an protein. Immunohistochemistry immunoelectron microscopy showed staining podocytes, was both intracellular plasma membrane. Conclusion. conclude that not expressed appreciably under conditions, following injury it significantly up-regulated, mainly