Glycosphingolipid receptors for anti-Gd and anti-p cold agglutinins

摘要: Abstract Anti-Gd and anti-p cold agglutinins exhibit similar serological properties: neuraminidase treatment of erythrocytes greatly reduces their agglutinability by these antibodies protease enhances agglutination. We reported previously that an agglutinin was inhibited sialosyllactoneotetraosylceramide, NeuAc(α2–3)Gal(β1–4)GlcNAc(β1–3)Gal(β1–4)Glc-Cer, the most abundant ganglioside human eryhrocytes. now report two less gangliosides are more potent inhibitors this antibody, anti-Gd antibodies, than sialosyllactoneotetraosyl-ceramide. These have same carbohydrate chain, NeuAc(α2–3)Gal(β1–4)GlcNAc(β1–3)Gal(β1–4) GlcNAc(β1–3)Gal(β1–4)Glc (SNH), but they differ in ceramide moiety. The principal fatty acid SNH-1 is C 16:0 , whereas SNH-2 contains a predominance 22:0 24:0 24:1 . No inhibition produced ganglioside, NeuAc(α2–6)Gal(β1–4)GlcNAc(β1–3)Gal(β1–4)Glc-Cer. Another monoclonal agglutinin, Sa, which shares some properties with agglutinins, not any gangliosides.