Molecular imprinted polymer for solid‐phase extraction of flavonol aglycones from Moringa oleifera extracts
作者： Vusumzi Pakade , Ewa Cukrowska , Sofia Lindahl , Charlotta Turner , Luke Chimuka
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摘要: Molecular imprinted polymer produced using quercetin as the imprinting compound was applied for extraction of flavonol aglycones (quercetin and kaempferol) from Moringa oleifera methanolic extracts obtained heated reflux method. Identification quantification these flavonols in achieved high performance liquid chromatography with ultra violet detection. Breakthrough volume retention capacity molecular SPE investigated a mixture myricetin, kaempferol. The calculated theoretical number plates found to be 14, 50 8 kaempferol, respectively. Calculated adsorption capacities were 2.0, 3.4 3.7 μmol/g No myricetin observed methanol extracts. Recoveries kaempferol leaves flowers ranged 77 85% 75 86%, respectively, demonstrating feasibility developed molecularly method quantitative clean-up both flavonoids. Using combined SPE, concentrations 975 ± 58 845 32 mg/kg determined flowers, However, 2100 176 2802 157 mg/kg,
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