Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens
作者： Franziska C. Eberle , Jeffrey C. Hanson , J. Keith Killian , Lai Wei , Kris Ylaya
DOI: 10.2353/JMOLDX.2010.090200
关键词:
摘要: Altered DNA methylation is a fundamental characteristic of carcinogenesis. The analysis in tumor cells may help to better understand pathogenesis and more importantly be used as diagnostic tool with therapeutic consequences. To detect targets relevant tumorigenesis, it essential separate neoplastic from nonneoplastic cells. An excellent method for isolating specific laser-assisted microdissection (LAM). Target cell identification immunoguided LAM (ILAM) requires immunohistochemistry (IHC). Yet, unclear whether IHC ILAM influences methylation. goals this study were establish an optimized protocol antigen retrieval formalin-fixed paraffin-embedded (FFPE) specimens suitable evaluate its effect on the methylome using high throughput array. Using ten archival FFPE specimens, we showed staining ILAM. Extracted microdissected immunohistochemically or HE P
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Lise Mette Gjerdrum, Ilze Lielpetere, Lars Melholt Rasmussen, Knud Bendix, Stephen Hamilton-Dutoit, Laser-Assisted Microdissection of Membrane-Mounted Paraffin Sections for Polymerase Chain Reaction Analysis : Identification of Cell Populations Using Immunohistochemistry and in Situ Hybridization The Journal of Molecular Diagnostics. ,vol. 3, pp. 105- 110 ,(2001) , 10.1016/S1525-1578(10)60659-9
Manel Esteller, Epigenetics in Cancer The New England Journal of Medicine. ,vol. 358, pp. 1148- 1159 ,(2008) , 10.1056/NEJMRA072067
Anna E. Szafranska, Timothy S. Davison, Jaclyn Shingara, Martina Doleshal, Judith A. Riggenbach, Carl D. Morrison, Scott Jewell, Emmanuel Labourier, Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling The Journal of Molecular Diagnostics. ,vol. 10, pp. 415- 423 ,(2008) , 10.2353/JMOLDX.2008.080018
Andrew P. Feinberg, Bert Vogelstein, Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature. ,vol. 301, pp. 89- 92 ,(1983) , 10.1038/301089A0
L Bhaw-Rosun, C Fleischmann, C A Mein, T Crook, P Smith, G Kelly, A Rosenwald, G Ott, E Campo, L M Rimsza, E B Smeland, W C Chan, N Johnson, R D Gascoyne, S Reimer, R M Braziel, G W Wright, L M Staudt, T A Lister, J Fitzgibbon, C O'Riain, D M O'Shea, Y Yang, R Le Dieu, J G Gribben, K Summers, J Yeboah-Afari, Array-based DNA methylation profiling in follicular lymphoma Leukemia. ,vol. 23, pp. 1858- 1866 ,(2009) , 10.1038/LEU.2009.114
Ronald J. Buckanovich, Dimitra Sasaroli, Ann O'Brien-Jenkins, Jeffrey Botbyl, José R. Conejo-Garcia, Fabian Benencia, Lance A. Liotta, Phyllis A. Gimotty, George Coukos, Use of immuno-LCM to identify the in situ expression profile of cellular constituents of the tumor microenvironment. Cancer Biology & Therapy. ,vol. 5, pp. 635- 642 ,(2006) , 10.4161/CBT.5.6.2676
Christine Ladd-Acosta, Jonathan Pevsner, Sarven Sabunciyan, Robert H. Yolken, Maree J. Webster, Tiffany Dinkins, Pauline A. Callinan, Jian-Bing Fan, James B. Potash, Andrew P. Feinberg, DNA Methylation Signatures within the Human Brain American Journal of Human Genetics. ,vol. 81, pp. 1304- 1315 ,(2007) , 10.1086/524110
J Rodriguez-Canales, JC Hanson, MA Tangrea, HS Erickson, PS Albert, BS Wallis, AM Richardson, PA Pinto, WM Linehan, JW Gillespie, MJ Merino, SK Libutti, KG Woodson, MR Emmert-Buck, RF Chuaqui, Identification of a unique epigenetic sub-microenvironment in prostate cancer The Journal of Pathology. ,vol. 211, pp. 410- 419 ,(2007) , 10.1002/PATH.2133
Juan Rosai, Why microscopy will remain a cornerstone of surgical pathology Laboratory Investigation. ,vol. 87, pp. 403- 408 ,(2007) , 10.1038/LABINVEST.3700551
Falko Fend, Michael R. Emmert-Buck, Rodrigo Chuaqui, Kristina Cole, Jeff Lee, Lance A. Liotta, Mark Raffeld, Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. American Journal of Pathology. ,vol. 154, pp. 61- 66 ,(1999) , 10.1016/S0002-9440(10)65251-0