Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil

摘要: The study of host associations mosquitoes (Diptera, Culicidae) provides valuable information to assist in our understanding a variety related issues, from their life-history the entomological surveillance pathogens. In this study, we identified and characterized mosquito blood meals both urban forested areas city Paranagua, state Parana, Brazil, by analyzing amplification DNA ingested under different storage conditions digestion levels. Host preservation was evaluated fresh according duration (30 180 days) temperature (-20°C / -80°C) and, digested blood, degree classified on Sella scale. Molecular analysis based extraction fragment mitochondrial COI gene. We determined that, up to180 days storage, temperatures did not influence DNA, whereas success increasingly reduced over course process. species Anopheles cruzii, Aedes fluviatilis, scapularis, Psorophora ferox, Culex quinquefasciatus, mollis, intrincatus, together with specimens representing four subgenera one genus Culicidae [Ae. (Ochlerotatus), Cx. (Culex), (Melanoconion), (Microculex), Limatus, respectively] had identified. Their diverse use evidenced identification 19 vertebrate host, namely two amphibians, three mammals 14 birds. Birds were most commonly meals. These results only show diversity hosts, but also underscore challenges involved monitoring arboviruses public health importance, given potential combinations for each species.