Usefulness of Whole-Blood Interferon-γ Assay and Interferon-γ Enzyme-Linked Immunospot Assay in the Diagnosis of Active Pulmonary Tuberculosis

摘要: Purpose: The aim of this study was to evaluate the usefulness whole-blood interferonassay (enzyme-linked immunosorbent assay [ELISA]) and interferon- enzyme-linked immunospot (ELISPOT) based on early secretory antigenic target 6 culture filtrate protein 10 in diagnosis active pulmonary tuberculosis (TB) routine clinical practice. Method: We conducted a prospective enrolling 144 participants with suspected TB tertiary referral hospital Seoul, South Korea, investigate diagnostic sensitivity, specificity, positive predictive value (PPV), negative (NPV) these tests. Clinical assessment, tuberculin skin test (TST), ELISA (QuantiFERON-TB Gold [QFT-G]; Cellestis Ltd; Victoria, Australia), an ELISPOT (T SPOT.TB; Oxford Immunotec; Oxford, UK) were performed. Test results compared final confirmed diagnoses. Results: Active diagnosed 67 (47%). Sensitivities QFT-G T SPOT.TB for 89% (95% confidence interval [CI], 79 96%) 92% CI, 83 97%), respectively; specificities 49% 37 61%) 47% 36 59%). NPVs (84%; 95% 69 93%) (87%; 73 higher than that TST (64%; 51 76%) [p 0.001 p < 0.001, respectively]. Conclusion: High suggest supplementary role tests exclusion TB, although low PPV limits their practice where prevalence latent infection is considerable. (CHEST 2007; 132:959–965)