Hydrogenated amorphous silicon ultraviolet sensor for deoxyribonucleic acid analysis
作者： G. de Cesare , D. Caputo , A. Nascetti , C. Guiducci , B. Riccò
DOI: 10.1063/1.2179608
关键词:
摘要: In this letter, we show the results achieved using a hydrogenated amorphous silicon photosensor for “label-free” deoxyribonucleic acid (DNA) analysis based on measurements of absorbance in ultraviolet range. The optimization sensor structure allowed us to bring detection limit 30-mer DNA sample down 1nM×cm, limited by experimental setup. Taking advantage hypochromic effect, also demonstrated single- and double-stranded molecules melting experiment. From noise characterization our setup, estimated minimum required detect occurrence hybridization/separation process be below 10−3.
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