Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium, Enterobacter lignolyticus

摘要: To process plant-based renewable biofuels, pretreatment of plant feedstock with ionic liquids has significant advantages over current methods for deconstruction lignocellulosic feedstocks. However, are often toxic to the microorganisms used subsequently biomass saccharification and fermentation. We previously isolated Enterobacter lignolyticus strain SCF1, a lignocellulolytic bacterium from tropical rain forest soil, report here that it can grow in presence 0.5 M 1-ethyl-3-methylimidazolium chloride, commonly liquid. investigated molecular mechanisms SCF1 liquid tolerance using combination phenotypic growth assays, phospholipid fatty acid analysis, RNA sequencing technologies. Potential modes resistance chloride include an increase cyclopropane acids cell membrane, scavenging compatible solutes, up-regulation osmoprotectant transporters drug efflux pumps, down-regulation membrane porins. These findings represent important first step understanding bacteria provide basis engineering microbial tolerance.