Sensitive liquid chromatography-mass spectrometry assay for quantitation of docetaxel and paclitaxel in human plasma

摘要: Abstract We have developed a high-performance liquid chromatography–electrospray ionization mass spectrometry (LC–MS) method for quantifying docetaxel and paclitaxel in human plasma. The assay fulfills the need defining lower plasma concentrations of these antineoplastic agents that result from number changes how are used clinically. uses as internal standard docetaxel, vice versa; solid-phase extraction; Phenomenex Hypersil ODS (5 μm, 100×2 mm) reversed-phase analytical column; an isocratic mobile phase 0.1% formic acid methanol–water (70:30, v/v); spectrometric detection using electrospray positive mode electron ionization. has limit quantitation (LLOQ) 0.3 nM is linear between 1 μM docetaxel. For paclitaxel, LLOQ was nM, μM. demonstrated suitability this by it to quantify patient given 40 mg/m2 comparing those results produced when same samples were assayed with HPLC absorbance detection. In similar manner, 15 LC–MS assay, which proved superior because its greater sensitivity relatively short (7 min) run time, should be important tool future pharmacokinetic analyses paclitaxel.