Suppression of pervasive noncoding transcription in embryonic stem cells by esBAF

摘要: Approximately 75% of the human genome is transcribed, majority which does not encode protein. However, many noncoding RNAs (ncRNAs) are rapidly degraded after transcription, and relatively few have established functions, questioning significance this observation. Here we show that esBAF, a SWI/SNF family nucleosome remodeling factor, suppresses transcription ncRNAs from ∼57,000 nucleosome-depleted regions (NDRs) throughout mouse embryonic stem cells (ESCs). We esBAF functions to both keep NDRs nucleosome-free promote elevated occupancy adjacent NDRs. Reduction upon depletion strongly correlated with ncRNA expression, suggesting flanking nucleosomes form barrier pervasive transcription. Upon forcing near two using nucleosome-positioning sequence, found no longer required silence Therefore, esBAF's function enforce NDRs, its maintain in state, necessary for silencing over ncDNA. Finally, ability positioned repress depends on translational positioning. These data reveal novel role suppressing open chromatin ESCs.