Ultrathin Polyimide Membrane as Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium
作者： Tanja Ilmarinen , Hanna Hiidenmaa , Peeter Kööbi , Soile Nymark , Anni Sorkio
DOI: 10.1371/JOURNAL.PONE.0143669
关键词:
摘要: In this study, we investigated the suitability of ultrathin and porous polyimide (PI) membrane as a carrier for subretinal transplantation human embryonic stem cell (hESC) -derived retinal pigment epithelial (RPE) cells in rabbits. The vivo effects hESC-RPE were analyzed by suspension injection into Royal College Surgeons (RCS) rats. Rat eyes with electroretinography (ERG) histology. After analyzing surface permeability properties PI, PI transplantations without performed rabbits followed three months fundus photography, ERG, optical coherence tomography (OCT), Animals immunosuppressed cyclosporine entire follow-up time. dystrophic RCS rats, ERG outer nuclear layer (ONL) thickness showed some rescue after injection. Cells positive antigen found clusters under retina 41 days post-injection but not anymore 105 days. rabbits, OCT good placement PI. However, there was loss pigmentation on hESC-RPE-PI over alone, no obvious signs inflammation or atrophy observed. presence hESC-RPE, mononuclear infiltration observed around membranes. well-tolerated space is promising scaffold RPE transplantation. rejection transplanted seems to be major problem given immunosuppression insufficient reduction xenograft induced inflammation.
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D Bok, J G Flannery, V M Clark, B A Pfeffer, Membrane receptors for retinol-binding protein in cultured human retinal pigment epithelium. Investigative Ophthalmology & Visual Science. ,vol. 27, pp. 1031- 1040 ,(1986)
Matthew M. LaVail, Legacy of the RCS rat: impact of a seminal study on retinal cell biology and retinal degenerative diseases. Progress in Brain Research. ,vol. 131, pp. 617- 627 ,(2001) , 10.1016/S0079-6123(01)31048-8
Hiroyoshi NINOMIYA, Tomo INOMATA, Nobuyuki KANEMAKI, ウサギの眼の微細血管系 : 血管樹脂鋳型標本の走査型電子顕微鏡による観察(実験動物学) Journal of Veterinary Medical Science. ,vol. 70, pp. 887- 892 ,(2008) , 10.1292/JVMS.70.887
Susanna Narkilahti, Riitta Suuronen, Jari Hyttinen, Kati Juuti-Uusitalo, Hannu Uusitalo, Niina Onnela, Tanja Ilmarinen, Hanna Vaajasaari, Kristiina Rajala, Heli Skottman, Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells. Molecular Vision. ,vol. 17, pp. 558- 575 ,(2011)
Tongbiao Zhao, Zhen-ning Zhang, Peter D. Westenskow, Dilyana Todorova, Zheng Hu, Tongxiang Lin, Zhili Rong, Jinchul Kim, Jingjin He, Meiyan Wang, Dennis O. Clegg, Yong-guang Yang, Kun Zhang, Martin Friedlander, Yang Xu, Humanized Mice Reveal Differential Immunogenicity of Cells Derived from Autologous Induced Pluripotent Stem Cells. Cell Stem Cell. ,vol. 17, pp. 353- 359 ,(2015) , 10.1016/J.STEM.2015.07.021
Yalong Dang, Chun Zhang, Yu Zhu, Stem cell therapies for age-related macular degeneration: the past, present, and future. Clinical Interventions in Aging. ,vol. 10, pp. 255- 264 ,(2015) , 10.2147/CIA.S73705
Yuntao Hu, Laura Liu, Bo Lu, Danhong Zhu, Ramiro Ribeiro, Bruno Diniz, Padmaja B Thomas, Ashish K Ahuja, David R Hinton, Yu-Chong Tai, Sherry T Hikita, Lincoln V Johnson, Dennis O Clegg, Biju B Thomas, Mark S Humayun, None, A novel approach for subretinal implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium monolayer. Ophthalmic Research. ,vol. 48, pp. 186- 191 ,(2012) , 10.1159/000338749
Shaomin Peng, Veena S. Rao, Ron A. Adelman, Lawrence J. Rizzolo, Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium Investigative Ophthalmology & Visual Science. ,vol. 52, pp. 1392- 1403 ,(2011) , 10.1167/IOVS.10-5984
K Sugahara, J H Caldwell, R J Mason, Electrical currents flow out of domes formed by cultured epithelial cells. Journal of Cell Biology. ,vol. 99, pp. 1541- 1544 ,(1984) , 10.1083/JCB.99.4.1541
Vamsi K. Gullapalli, Ilene K. Sugino, Yancy Van Patten, Sumit Shah, Marco A. Zarbin, Impaired RPE survival on aged submacular human Bruch's membrane. Experimental Eye Research. ,vol. 80, pp. 235- 248 ,(2005) , 10.1016/J.EXER.2004.09.006