ERCC1 Expression Is a Predictor of Survival in Resected Patients With Non-small Cell Lung Cancer

摘要: Study objectives Proteins of the nucleotide excision repair pathway DNA damage. The cross-complementing ( ERCC ) gene family reduces damage to by and repair. Impaired nuclear could lead increased genomic instability that in turn a more malignant phenotypic behavior tumors. We therefore evaluated effect intratumoral ERCC1 expression on survival non-small cell lung cancer (NSCLC) patients who underwent surgical resection for cure Design Resected tumor corresponding normal specimens from 51 with NSCLC were immediately frozen liquid nitrogen. Total RNA was extracted, reverse transcribed, amplified intron-spanning primers. Quantitation done using Taqman procedure, normalized 18S rRNA as internal reference levels expressed unit-less ratio Results Tumoral ranged 4.96 2,008, median value 54.76. Using an 50 dichotomize cohort, there statistically significant difference > (94.6 months) compared Conclusions conclude resected high (> 50) have better when low