Isolation and structural characterisation of a propoxyphenyl-linked thiohomosildenafil analogue found in a herbal product.

摘要: A propoxyphenyl-linked thiohomosildenafil analogue, one of the sildenafil analogues, was found in an herbal product. It isolated by semi-preparative high-performance liquid chromatography (HPLC). The structure established based on a comparison chromatographic and spectroscopic behaviour with other analogues using HPLC diode array detection, quadrupole time-of-flight mass spectrometry (Q-TOF/MS), nuclear magnetic resonance (NMR) spectroscopy. analysis showed separation from known similar retention time. An [M + H](+) ion at m/z 519.22 detected corresponding to empirical formula C24H34N6O3S2. that thiohomosildenafil, except ethoxy group attached phenyl ring substituted for propoxy group. assigned as 5-[2-propoxy-5-(4-ethylpiperazin-4-ylsulfonyl)phenyl]-3-methyl-1-n-propyl-4,5,dihydro-1H-pyrazole[7,1,d]pyrimidin-4-thione named propoxyphenyl-thiohomosildenafil because considerably thiohomosildenafil.