Antibodies to β2-Glycoprotein-I: Urea Resistance, Binding Specificity, and Association with Thrombosis

摘要: The aim of the present study was to evaluate urea resistance and binding characteristics anti-β2-glycoprotein I (anti-β2GPI) antibodies using standard anticardiolipin (aCL) anti-β2GPI enzyme immunosorbent assays (ELISAs). Sera from patients with antiphospholipid syndrome (APS) (n = 22) non-APS 24), positive in a aCL ELISA, were tested an ELISA performed polystyrene-irradiated plates. Urea ELISAs by measuring ability recognize antigen presence 2 M urea. serum dilution after treatment (D) expressed as percentage without (Do) corresponding same optical density defined residual activity (RA 100 D/Do). higher RA, APS compared sera had (absorbance values ranging between 0.180 1.400; median, 0.717 vs 0.120–1.273; 0.250, respectively; P < 0.004). Six patients' low levels but they RA ≥30%. Anti-β2GPI detected 15 22 3 24 (P 0.03); ≥30% 1 23 Using CL affinity column, purified three negative anti-β2GPI-positive highly bovine albumin (BSA) blocking agent (modified ELISA); reactivity not two one sera. On contrary, high when β2GPI incubated plates; thus some recognized CL/β2GPI complex, rather than or alone. In conclusion, anti-β 2GPI are common patients, number such complex β2GPI. Antibodies either derived exist minority autoimmune disease.