How Molecular Motors Are Arranged on a Cargo Is Important for Vesicular Transport

摘要: The spatial organization of the cell depends upon intracellular trafficking cargos hauled along microtubules and actin filaments by molecular motor proteins kinesin, dynein, myosin. Although much is known about how single motors function, there significant evidence that in vivo are carried multiple motors. While some aspects function have received attention, cargo itself —and on cargo—affects transport has not been considered. To address this, we developed a three-dimensional Monte Carlo simulation transporting spherical cargo, subject to thermal fluctuations produce both rotational translational diffusion. We found these could exert load motor(s), significantly decreasing mean travel distance velocity large cargos, especially at viscosities. In addition, presence dramatically help bind productively microtubule: relatively slow diffusion moderately sized gave ample opportunity microtubule before motor/cargo ensemble diffuses out range microtubule. For rapidly diffusing probability their binding was high if were nearby they easily reach Our simulations one reason why may be approximately 100 nm long improve ‘on’ rates when attached comparably cargos. Finally, our results suggested efficiently regulate number active motors, should clustered together rather than spread randomly over surface cargo. uses specific parameters for effects result from generic properties filaments, so apply other as well.