A model of human cutaneous candidosis based on reconstructed human epidermis for the light and electron microscopic study of pathogenesis and treatment

摘要: The use of reconstructed human epidermis provided the basis for an in vitro model cutaneous candidosis. Candida albicans blastospores on surface provoked following changes within 72 h: superficial keratin degradation, scaling, hyperkeratosis, parakeratosis, dyskeratosis representing hyperproliferative stress, spongiosis, and vesiculation. Great differences intensity these reactions intact or chemically mechanically damaged illustrate importance stratum corneum as a barrier. Uninfected showed prominent cell proliferation wound healing h after mechanical chemical pretreatment. These signs repair were blocked presence C. able to invade corneum. When desmosomes accessible, high affinity structures was observed. A single application econazole liposome dispersion decreased hyperkeratosis dyskeratosis. Morphological alterations treatment with proposed identical, described established animal models. This disease may provide insight into pathogenesis candidosis substitute models investigations humans.