Odontogenic ameloblast-associated and amelotin are novel basal lamina components

摘要: Odontogenic ameloblast-associated (ODAM) and amelotin (AMTN) are secreted by maturation stage ameloblasts accumulate at the interface with enamel where an atypical basal lamina (BL) is present. This study aimed determining quantifying ultrastructural distribution of ODAM AMTN cell–tooth interface. Ultrathin sections organs from early to mid- late amelogenesis were processed for immunogold labeling antibodies against ODAM, or lectins wheat germ agglutinin, Helixpomatia agglutinin (HPA) Ricinus communis I agglutinin. Immunolabeling showed that both localized BL. Quantitative analyses indicated beginning there a concentration on cell side BL while appears more concentrated side. In stage, such differential no longer apparent. All three bound Competitive incubation native did not affect binding efficiency ODAM; however, was significantly reduced after HPA. conclusion, bona fide components associated they organize into different subdomains during stage. The data also suggest dynamic structure rearranges its organization as advances. Finally, abrogation antibody HPA supports presence O-linked sugars in molecule and/or close association other O-glycosylated molecules.