S100A4 siRNA inhibits human pancreatic cancer cell invasion in vitro.
作者： Feng Sheng Li , Mao Min Song , Li Hui Liu , Xiao Hua Chen , Na Li
DOI: 10.3967/0895-3988.2012.04.012
关键词:
摘要: Abstract Objective Pancreatic cancer is one of the most deadly cancers, which characterized by its high metastatic potential. S100A4 a major prometastatic protein involved in tumor invasion and metastasis precise role pancreatic has not been fully investigated. We knocked down gene Bxpc-3 cell line via RNA interference to study changes behavior. Methods Real-time polymerase chain reaction western blotting were used detect mRNA expression levels S100A4, matrix metalloproteinase (MMP)-2, E-cadherin thrombospondin (TSP)-1. Transwell chambers migration abilities; adhesion assay was ability; colony forming efficiency proliferation; flow cytometry apoptosis. Results reduced 17% after transfection with S100A4-siRNA, had similar trend. MMP-2 that TSP-1 elevated, indicating affects their expression. S100A4-silenced cells exhibited marked decrease invasiveness increased adhesion, whereas overall proliferation apoptosis overtly altered. Conclusion downstream factors play important roles invasion, silencing A100A4 can significantly contain cancer.
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