Aberrant CpG Island Hypermethylation of Chronic Gastritis, in Relation to Aging, Gender, Intestinal Metaplasia, and Chronic Inflammation

摘要: Aberrant hypermethylation of promoter CpG islands is an important mechanism for the inactivation tumor suppressor genes. island occurs in relation to tumorigenesis or aging. Gastric cancer one tumors with a high level aberrant methylation. However, data on methylation status normal gastric mucosa has been very limited. The present study attempted compare nonneoplastic mucosa, using clinicopathological parameters, including age, gender, Helicobacter pylori (H. pylori), acute and chronic inflammation, intestinal metaplasia. Two hundred sixty-eight samples were studied 11 genes (COX-2, DAP-kinase, E-cadherin, GSTP1, MGMT, hMLH1, p14, p16, THBS1, TIMP3, RASSF1A), methylation-specific PCR. was found 53.7, 41, 37.7, 23.1, 18.7, 10.9, 10, 4.1, 3.4, 1.7, 0.4% COX-2, hMLH1 RASSF1A, respectively. Five (DAP-kinase, TIMP-3) showed general progressive increase frequency as function aging, whereas other RASSF1A) rarely methylated. Male patients higher numbers methylated than females (3.2 vs. 2.1, respectively, P = 0.002). Gastritis marked metaplasia, those without (3.7 2.6, 0.021). infiltration mononuclear cells displayed mild moderate (3.4 2.5 2.5, < 0.05). Our results demonstrated that many are stomach suggested male inflammation closely associated increased samples.