Rotavirus vaccine administered parenterally induces protective immunity

摘要: We performed experiments to determine whether parenteral immunization with SA11 rotavirus can induce active protective immunity in a rabbit model of infection. After one or two intramuscular injections 1 ml live formalin-inactivated virus, we evaluated the mucosal and serologic immune response protection from challenge high dose live, virulent (Ala) rotavirus. Inactivated virus preparations, by enzyme-linked immunosorbent assay (ELISA) panel VP4- VP7-specific neutralizing nonneutralizing monoclonal antibodies, did not show loss epitopes inactivation procedure compared virus. Administration doses vaccine, at zero days postvaccination (DPV) booster shot 49 DPV, followed 71 DPV 3.5 x 10(5) PFU Ala resulted challenge. None two-dose virus-vaccinated rabbits shed after challenge, while shedding was detected all control (P = 0.001, Fisher's exact two-tailed test). Differences total serum immunoglobulin (Ig) antirotavirus ELISA titers < 0.05, Wilcoxon's rank sum test) were observed between groups vaccinated aluminum phosphate Freund's adjuvant but inactivated either adjuvant. All given vaccine had detectable intestinal antibody IgG, IgA, isotype. fourfold greater increases IgG responses three rabbits, whereas controls an IgA response. In contrast, vaccination 21 protect challenge; no difference mean number any observed. A serologic, mucosal, one-dose regimen. groups. These data indicate that two, one, challenge.(ABSTRACT TRUNCATED AT 400 WORDS)