Macromolecule-ligand binding studied by the Hummel and Dreyer method: current state of the methodology.

摘要: The use of the Hummel and Dreyer method to measure binding parameters ligand-macromolecule associations is reviewed. possibility determine number sites their association constants, even in case low affinity, control free ligand concentration as an independent variable are main advantages method. conditions validity rapid equilibrium kinetics, independence between macromolecule association, identical retention rates bound macromolecules. Initially developed on soft gels, has been applied high-performance chromatography capillary zone electrophoresis. Technical progress such increase resolution, detection sensitivity, automation have improved its utilization. given by general similar those obtained other techniques, comparable experimental (equilibrium dialysis, ultrafiltration, frontal elution, vacancy peak method, affinity electrophoresis, analysis, physical methods). choice these methods directed material availability practical constraints. Separation new types chromatographic columns or electrophoresis would enable study simultaneous different drugs same competition.