Expression of vimentin and glial fibrillary acidic protein in ethylnitrosourea-induced rat gliomas and glioma cell lines.
作者： G. Reifenberger , T. Bilzer , R. J. Seitz , W. Wechsler
DOI: 10.1007/BF00687757
关键词:
摘要: The expression of glial fibrillary acidic protein (GFAP) and vimentin was investigated immunohistochemically in 104 experimental gliomas induced by transplancental application ethylnitrosourea (ENU) CDF rats. Immunoreactivity for prominent many astrocytic tumor cells especially small glioma forming anaplastic medulloblastoma-like foci tumors. majority oligodendroglial tumors were negative, except some the large polymorphous oligodendrogliomas which contained intermingled positive cells. GFAP immunoreactivity detectable only a low fraction astrocytes few exceptional cases stained positive. Immunohistochemistry with antibodies against neurofilaments cytokeratins revealed no staining ENU-induced gliomas, while all oligodendrogliomatous HNK-1. Immunocytological immunoblot investigations two rat cell clones RG2 F98, are both derived from showed monolayer cultures syngeneic intracerebral transplantation F98 additionally demonstrated confluent RG2, on other hand, exhibited virtually culture but individual Our results more precise picture cellular differentiation widely used lines. They underline heterogeneity may comprise at different stages towards or astroglial phenotype.
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