The MqsR/MqsA toxin/antitoxin system protects Escherichia coli during bile acid stress.

摘要: Summary Toxin/antitoxin (TA) systems are ubiquitous within bacterial genomes, and the mechanisms of many TA well characterized. As such, several roles for have been proposed, such as phage inhibition, gene regulation persister cell formation. However, significance these is nebulous due to subtle influence from individual systems. For example, a single system has only minor contribution Hence, there lack defining physiological In this study, phenotype assays were used determine that MqsR/MqsA type II Escherichia coli important growth tolerance during stress bile salt deoxycholate. Using transcriptomics purified MqsR, we determined endoribonuclease toxin MqsR degrades YgiS mRNA, which encodes periplasmic protein promotes deoxycholate uptake reduces exposure. The importance reducing mRNA by evidenced improved growth, reduced death membrane damage when cells without ygiS stressed with Therefore, propose physiologically E. coli thrive in gallbladder upper intestinal tract, where high concentrations prominent.