ZAP70 in B-CLL cells related to the expression in NK cells is a surrogate marker for mutational status.

摘要: The strongest prognostic factor in chronic B-cell lymphocytic leukemia (CLL) is the mutational status of immunoglobulin heavy chain variable region (IGHV) genes. Determination this laborious and therefore not applied routine diagnostics. A search for “surrogate markers” has been conducted over past few years. One most promising surrogate markers ZAP70, but standardization measurement ZAP70 proven to be difficult. Conventionally, expression CLL cells related T cells. We propose a new method which NK used as reference (new NK-MFI method). We have measured samples 45 previously untreated patients. correlated better cytogenetic risk profile than conventional methods. Negativity both method) CD38 resulted probability 90% mutated IGHV In conclusion, marker cell © 2013 International Clinical Cytometry Society