Growth of Catharanthus roseus cell suspensions in bioreactors: On-line analysis of oxygen and carbon dioxide levels in inlet and outlet gas streams

摘要: Catharanthus roseus cells (C87N) grown in a 30 litre airlift vessel achieved growth rate of 0.366 day−1. The maximum biomass yield (9.13 gl−1) was recorded after 168 hours (7 days). On-line analysis the composition inlet and outlet gas streams during cycle allowed calculation metabolic activity cultures. Oxygen uptake on dry weight basis reached 4.5×10−4 Moles O2 g weight−1 h−1 96 (during mid-logarithmic phase growth) 2.7×10−3 l−1 volume (towards end logarithmic phase). Carbon dioxide production ran parallel with oxygen use maxima at 4.2×10−4 CO2 3.4×10−3 respectively.