Identification and localization of glucagon-related peptides in rat brain

摘要: Abstract Immunochemical and immunocytochemical techniques have been used to identify characterize glucagon-related peptides of the rat central nervous system. These show immunoreactivity with antiglucagon sera directed towards portion hormone, but not antisera specific for free COOH terminus glucagon. Highest concentrations were found in hypothalamus (6.1 +/- 1.6 ng/g wet weight) although lower amounts (approximately 2 ng/g) cortex, thalamus, cerebellum, brain stem. Gel filtration extracts revealed at least two immunoreactive forms, which molecular weights about 12,000 8000. Both had radioimmunoassay dilution curves parallel curve glucagon both identical counterparts intestine. Digestion intestinal trypsin plus carboxypeptidase B released COOH-terminal tryptic fragment pancreatic from these larger forms. Immunocytochemical studies using serum peroxidase-antiperoxidase staining identified glucagon-like material neuronal cell bodie processes magnocellular paraventricular nucleus, as well scattered cells supraoptic nucleus fibers median eminence. results suggest that glucagon-containing undergone type posttranslational modification are present hypothalamus.