Identification and Characterization at the Single-Cell Level of Cytokine-Producing Circulating Cells in Children With Dengue.

摘要: In this study, we identified, at the single-cell level, naturally induced cytokine-producing circulating cells (CPCCs) in children with dengue virus (DENV) infection ranging clinically from mild to severe disease. Tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) CPCCs were detected primary or secondary acute infection, pattern of these cytokines was similar that seen supernatant cultured peripheral blood mononuclear partially comparable found plasma. Monocytes, B cells, myeloid dendritic (mDCs) detected, frequency mDCs significantly higher isolated spontaneously secreted TNF-α, IL-6, 10, supernatants cultures purified activation allogeneic T supporting an antibody-independent function during DENV infection. Thus, could be a new immune parameter potential use evaluate pathogenesis